ABSTRACT

Cerebral vasospasm is delayed-onset cerebral arterial narrowing in response to blood clots left in the subarachnoid space after spontaneous aneurysmal subarachnoid hemorrhage (SAH). In the present study, we aimed to synthesize and investigate the cerebroprotective effects of formazans (quinazolinoneformazans – QF1 and QF2) for attenuation of subarachnoidhemorrhage induced cerebral vasospasm in rats. Subarachnoid hemorrhage (SAH) was performed by using a blood injection method. The neurological status of all animals was evaluated prior to surgery and 24hr post-surgery before euthanization and brain tissue harvesting. The brains were subjected for histopathological analysis. The cerebral vasospasm was noted measuring the diameter of major carotid artery (MCA) segment and the values from SAH and Sham animals were compared.All animals recovered from surgery and returned to their baseline activity with no motor weakness on spontaneous gait. There was no indication that the procedure caused any significant damage to the brain stem. Histological examination showed normal arterial wall features in control animals. Animals with SAH demonstrated significantly corrugated and thickened wall structure with decreased diameter on cross-section in SAH + vehicle group were significantly different from the sham group. The diameter was attenuated whereas the thickness was increased in the SAH + vehicle group as compared with the sham group. Simvastatin had shown great changes in these parameters while formazans exhibited mild changes in the parameters compared to SAH + Vehicle.From the results obtained the current work demonstrated that the synthesized formazans (QF1 and QF2) does not exhibit motor weakness on spontaneous gait. From the histopathological study the formazans have limited significance in attenuation of SAH induced cerebral vasospasm. Keywords: Subarachnoid Hemorrhage (SAH), Cerebral vasospasm, Simvastatin, Formazans, Major carotid artery
Publication date: 01/12/2021

https://ijbpas.com/pdf/2021/December/MS_IJBPAS_2021_5760.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.12.5760