ABSTRACT

Objective: The current line of investigation was focused at perusing the in-vitro anti-oxidant potential and anti –urolithiatic activities of aqueous extract of Nigella sativa seeds using various methods Materials and Methods: The dried seed powder was subjected to maceration method to result crude aqueous extract. Phytoconstituents present in aqueous extract was examined by performing preliminary phytochemical screening. Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total alkalodal content (TAC) and Antioxidant potential for crude extracts were studied by DPPH, FRAP and Total anti-oxidant assay method methods. Anti-urolithiatic activity was screened using nucleation and aggregation assays in-vitro. Results: The presence of phytochemical constituents, investigation of total phenolic, flavonoid content and total alkaloid content of aqueous extract of Nigella sativa seeds using various in-vitro assays were studied. The total phenolic content and flavonoid content of aqueous extract of plant was found to be 95.23 ±0.629 mg and 29.46 ±1.170 mg of GAE and Quercetin equivalents respectively. Total alkaloidal content was found to be 153mg/g of extract. The aqueous extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH), ferric reducing antioxidant power assays (FRAP) and Total antioxidant assay methods. Anti urolithiatic activity was studied using nucleation and aggregation assays. Conclusion: From the findings of current study, it was revealed that the selected medicinal plant Nigella sativa has good potential to treat urolithiasis. Keywords: Nigella sativa, Antioxidant activity, Aggregation assay, Nucleation assay
Publication date: 01/03/2026

https://ijbpas.com/pdf/2026/March/MS_IJBPAS_2026_9916.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.3.9916