ABSTRACT

The present study was designed to investigate the nephroprotective effect of Hemigraphis colorata (Blume) leaf extract both in-vitro and in-vivo. The in-vitro study examined the cell viability by using HEK 293 cells by MTT assay and investigated the role of Hemigraphis colorata in modulating this effect. This study also evaluated the in-vivo effect of Hemigraphis colorata against gentamicin induced nephrotoxicity in rats by studying the biochemical parameters and histopathological changes. The estimation of biochemical parameters like serum creatinine, serum urea and blood urea nitrogen were done using the serum. Gentamicin administration showed nephrotoxicity by a significant elevation in BUN, creatinine, urea and urine volume than normal range along with histopathological abnormalities. Treatment with Hemigraphis colorata normalized almost all the parameters of gentamicin induced nephrotoxicity in rats. The above findings were confirmed by histopathological examinations. Results of the present study suggests that Hemigraphis colorata has a significant effect on protecting kidneys against gentamicin induced nephrotoxicity. This protective effect could be attributed to the presence of flavonoids and phenolic compounds such as ferulic and gallic acid which is present in the plant. Therefore, Hemigraphis colorata has the potential to be developed as a good nephroprotective. Keywords: Hemigraphis colorata, Gentamicin, Nephrotoxicity, Antioxidant, MTT Assay
Publication date: 01/03/2026

https://ijbpas.com/pdf/2026/March/MS_IJBPAS_2026_9271.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.3.9271