ABSTRACT

The detection of hormone residues and steroids in food animals, particularly chicken, has grown into a global concern for food security. The current review's objective was to thoroughly examine the analytical methods that are already in use, including extraction procedures and threshold concentrations. To highlight general patterns and method variability, a description about multi-residue analytical methods is presented. Steroid hormones can be identified using these approaches, which also involve the extraction of samples with purification, chromatography separation, as well as several detection methods. In order to evaluate the existence of steroids and along with hormone residues, a plurality of monitoring and detection practices have recently been transformed, such as chromatographic techniques, PCR, ELISA, biosensors, and assays. The potential of innovative analytical techniques is also covered. This review finishes with a summary of current developments and also future directions for the advancement of steroid analysis methodologies. Keywords: Steroid hormones, Advanced techniques, Extraction methods, Detection technologies and Multi-residue analysis
Publication date: 01/02/2026

https://ijbpas.com/pdf/2026/February/MS_IJBPAS_2026_9779.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.2.9779