ABSTRACT

The primary objective of this study is to evolve and verify a reliable, simple, precise, and convenient UV-visible spectroscopic method in order to determine the quantity of Topiroxostat, following the recommendations outlined in ICH Q2 (R1). The multivariate calibration uses linear regression analysis to establish the association of concentration with absorbance at five specific wavelengths that are evenly distributed. Using ethanol as the solvent, Topiroxostat displayed ????max at 272 nm. A linear plot was obtained with a regression coefficient 0.999 for the concentrations between 8-12 ?g mL-1. The % RSD for inter-day and intra-day precision was found to be 0.285 and 0.234, respectively, and the assay was determined to be 99.52 % w/w. Keywords: ICH guideline, UV-visible spectrophotometry, Topiroxostat, Multivariate calibration
Publication date: 01/01/2026

https://ijbpas.com/pdf/2026/January/MS_IJBPAS_2026_9739.pdf

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https://doi.org/10.31032/IJBPAS/2026/15.1.9739