ABSTRACT

The purpose of this study is to develop a simple, quick, and reproducible reverse phase high performance liquid chromatography (RP-HPLC) technique for estimating Vildagliptin in both pure and tablet dose form utilizing an Agilent Eclipse XDB-C18, 4.6 x 150 mm, 5 ?m column. The mobile phase is made up of mixture of phosphate buffer and acetonitrile in the ratio of 85:15% v/v. The detection was performed at 210 nm, and the calibration curve was linear in the concentration range of 10-150 ?g/mL. The approach was statistically verified in terms of linearity, precision, accuracy, stability, specificity, LOD, and LOQ. The suggested RP-HPLC technique may be utilized to determine Vildagliptin in pure form because of its simplicity, speed, high precision, and accuracy. Keywords: Vildagliptin, RP-HPLC, mobile phase, flow rate, linearity, validation
Publication date: 01/01/2026

https://ijbpas.com/pdf/2026/January/MS_IJBPAS_2026_9662.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2026/15.1.9662