ABSTRACT

Bemotrizinol and Bisoctrizole is used as Sunscreen active ingredient. Sunscreen has been proven to decrease the development of skin cancer? It helps to prevent facial brown spots and skin discolorations. The present work was aim to develop and validate RP-HPLC method for the estimation of Bemotrizinol and Bisoctrizole in cream dosage form using Syncronis C18 (250mm × 4.6 mm) (5?m) as column and flow rate 1.2 ml/min. The whole mobile phase consist of 1,4 Dioxane: Ammonium Acetate buffer (80:20 v/v) and detection was carried out at 340 nm. The retention time for Bemotrizinol was 14.23 min and Bisoctrizole 11.00 min. The linearity range was found to be 10-30 ?g/ml (r2=0.9995) for Bisoctrizole and 20-100 ?g/ml (r2 =0.9984) for Bemotriznol. The limits of detection for Bemotrizinol and Bisoctrizole were 0.18 and 0.64 ?g/mL, respectively. The limits of quantification for Bemotrizinol and Bisoctrizole were 0.55 and 1.93 ?g/mL, respectively. The assay was found within the range of 98-102%. A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Bemotrizinol and Bisoctrizole. The method was successfully applied for the estimation of Bemotrizinol and Bisoctrizole in cream dosage form. Keywords: Bemotrizinol, Bisoctrizole, Validation, RP-HPLC
Publication date: 01/12/2025

https://ijbpas.com/pdf/2025/December/MS_IJBPAS_2025_9661.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2025/14.12.9661