ABSTRACT

The current study focuses on creating a unique methodology and testing it for the quantification of Febuxostat (FBX) in formulations and bulk. The primary objective of this research work was to develop a facile, precise, responsive, and validated UV spectrophotometric assay for Febuxostat utilizing the multivariate regression approach. This multivariate calibration technique relied upon equations generated from a linear regression model based on the mathematical relationship between absorbance and concentration at five equidistant wavelengths. Febuxostat exhibited a maximum absorption at a wavelength of 314 nm. The findings underwent statistical analysis to determine their significance. A linear plot exhibiting a regression coefficient of 0.999 was obtained for concentrations ranging from 4 to 6 ?g mL-1. The assay reported to be 99% to 101.13% w/w. Keywords: Febuxostat, Anti- gout agent, Multivariate calibration, UV spectrophotometry, Assay, Pharmaceutical formulations, ICH guidelines
Publication date: 01/12/2025

https://ijbpas.com/pdf/2025/December/MS_IJBPAS_2025_9600.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.12.9600