ABSTRACT

In this research, a simple, accurate and validated Ultra-violet spectrophotometric method for determination of Fluconazole using multivariate regression was developed. The calibration process implemented here is based upon equations obtained from linear regression using absorbance data collected at 5 equidistant wavelengths to establish the correlation of concentration and observed spectral characteristics. The maximum absorbance of Fluconazole was found to be at 261 nm. Results were tested for statistical significance. In a range of concentration between 100-300 ?g mL-1, a linear plot with the regression coefficient of 0.9992 was acquired. The assay was performed and found to be 99.75% - 100.04% w/w. Keywords: Fluconazole, UV spectrophotometry, Multivariate calibration, ICH guidelines
Publication date: 01/12/2025

https://ijbpas.com/pdf/2025/December/MS_IJBPAS_2025_9598.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.12.9598