ABSTRACT

The current study suggests creating a unique methodology and testing it to determine the amount of clomipramine in formulations and bulk. Sensitive, precise, and quick describe this analytical method. The analytical approach was carried out with the aid of a UV spectrophotometer and a multivariate calibration methodology. This multivariate calibration approach was developed using the link between concentration and absorbance, which has been established at five distinct wavelengths, including the absorption maxima at 252 nm. The procedure was validated using ICH Q2 (R1) criteria by using the linear regression equations as well as other mathematical and statistical methods. The validation parameters for accuracy, linearity, and others mentioned in the instructions have all been fulfilled. When used in routine examination of medications and formulations, this technology is sensitive, cost-effective, and yields reliable results. The technique greenness values were calculated using the analytical Eco scale, Agree metrics, and Green Analytical Procedure Index. Keywords: Clomipramine, Multivariate calibration technique, UV spectrophotometric, Pharmaceutical formulations, ICH guidelines, Validation
Publication date: 01/12/2025

https://ijbpas.com/pdf/2025/December/MS_IJBPAS_2025_8345.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2025/14.12.8345