The "friendly" bacteria Lactobacilli generally inhabit our gastrointestinal, urinary, and genital
systems without causing any harmful diseases. In this research, Lactobacillus strains were
isolated, characterized, and screened with potential probiotic attributes from sources like fruits
& vegetables, dairy & non-dairy products, fermented foods, cow milk, pineapples waste,
human breast milk, etc. Antimicrobial effect for both gram-positive & gram-negative
pathogens was tested. The samples were collected from 10 different sources from the local
areas of Rajkot, Gujarat, India. After growth on MRS agar medium, 42 bacterial isolates were
obtained, and pure cultures were also obtained by subculturing them on the same MRS
medium. The purity of the isolated Lactic acid bacteria was verified by morphological analysis,
Gram staining, and further identified by some Lactobacillus specific biochemical tests.
Considering the influence of pH, bile salt, and NaCl, isolated Lactobacillus strain exhibits
tolerance at lower pH (2.0), bile salt (up to 4%) and NaCl (up to 6%). Finally, based on their
phenotypic characteristics and molecular identification by 16S rDNA sequencing, two bacterial
isolates were identified as Lactobacillus spp. The two isolated strains (Limosilactobacillus
fermentum DBM2 and Lactiplantibacillus plantarum SDPW25) showed strong in vitro
antibacterial activity along with potential probiotic properties. Therefore, the current studyrepresents the isolation of Lactobacilli from different sources, their proposed mechanism as
probiotics, and the health benefits to human beings, along with their future aspects.
Keywords: Probiotics, Lactobacillus, isolation, screening, identification
Publication date: 01/10/2025
https://ijbpas.com/pdf/2025/October/MS_IJBPAS_2025_9489.pdf
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https://doi.org/10.31032/IJBPAS/2025/14.10.9489
