ABSTRACT

Lisinopril and Amlodipine are used for treatment of hypertension. This research aims to develop a robust and environmentally friendly method for the simultaneous determination of Lisinopril and Amlodipine through spectrophotometric derivatization using absorption correction techniques. The method involves use of ninhydrin as derivatizing agent in presence of NaHCO3. Absorption correction method involves estimation of Lisinopril and Amlodipine at 320 nm after derivatization and estimation of amlodipine without derivatization at 360 nm within the linear range of 20-160 ?g/ml. The results demonstrated the feasibility and accuracy of the proposed method, with validation according to ICH guidelines showing precision, accuracy over the linear range. The AGREE software was used to evaluate the greenness of the method, showing alignment with the principles of green analytical chemistry. The findings highlight the potential of the developed method for routine pharmaceutical analysis. Keywords: Spectrophotometry, Lisinopril, Amlodipine, Derivatization, Absorption correction, Green analytical chemistry
Publication date: 01/09/2025

https://ijbpas.com/pdf/2025/September/MS_IJBPAS_2025_9443.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.9.9443