The objective of the current study is to evaluate the phytochemical profile and cytotoxic properties
of the crude aqueous extract of Phaseolus vulgaris Linn. leaves. This plant is well-known for its
nutritional and agricultural benefits, and its crude extract was obtained through the sequential
extraction method. The extract was then subjected to High-Resolution Liquid Chromatography-
Mass spectrometry (HR-LCMS) analysis to identify the phytoconstituents present. A total of 120
phytoconstituents were obtained, with 26 compounds being identified as major constituents in both
positive and negative ESI modes. Sapropterin, Melibiose, Pirbuterol, Osmaronin, N-
Isovalerylglycine methyl ester, Beta-D-Galactopyranosyl-(1->4) -beta-D-galactopyranosyl- (1-
>4) -D-galactose, 3-Methyladenine, 3-Beta, 6-Beta-Dihydroxynortropane, D-pipecolic acid,
Lotaustralin, 8-Hydroxyadenine, L-threo-3-Phenylserine, L-isoleucyl-L-proline, Gentianadine, L-
Arogenate, Prolyl-Arginine, Norcotinine, Ganolucidic acid E, Benzocaine, Succinoadenosine, D-
Galactonate, Melibiose, Thiolactomycin, (Z)-5-[(5-Methyl-2-thienyl)methylene] -2(5H)
Furanone, Rutin, and Kuwanon Z. The toxicity of crude extracts at various concentrations wasevaluated using the Sulforhodamine B assay on the HaCaT cell line. The results indicated that the
IC50 value for the crude extract was 853.2 ± 0.11 g/ml. A dose-dependent cytotoxic effect of the
crude aqueous extract was observed on the HaCaT cell line. Therefore, it is necessary to isolate,
purify, and characterize bioactive compounds responsible for their bioactivity.
Keywords: HR-LCMS, HaCaT cell line, Leaves, Phaseolus vulgaris Linn
Publication date: 01/09/2025
https://ijbpas.com/pdf/2025/September/MS_IJBPAS_2025_9435.pdf
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https://doi.org/10.31032/IJBPAS/2025/14.9.9435
