ABSTRACT

Aim: For the purpose of analyzing the active principle in the obtained extracts of the herbs, GC-MS is considered as an important tool. This study includes two techniques where the components is separated by Gas chromatography and the mass spectroscopy does the analysis of the active component. As a part of such an analytical aspect, Shoolaprashaman Churna is subjected to GC-MS to study their bio active compounds. Methodology – 1grm of sample was extracted with 10 ml of methanol and was filtered through a syringe filter (Nylon 13mm 0.2um) and injected 1ml /min at temp. Of 260oC at GCMS. The components of the oil were identified by comparison of their mass spectra with those of the spectrometer/mass spectral database using NIST Library (NIST-08 SPECTRAL DATA). The identifications were confirmed by comparison of fragmentation pattern and their retention indices with those reported in literature.Result: Result shows peaks with R.T mins as 11.052, 11.807, 11.942 and the compounds identified are Retrofractamide- A, C20H25O3N (2E, 4E,8E)-9-(Benzo [D], Dioxol-5-YL), (Piperidin-1-YL)Nona-2,-Trien-1-O,C21H25O3N , Trans-2-Methoxy-.Beta.-Methyl-.Beta.-Nitrostyrene 193 C10H11O3N 6306, Safrole , C10H10O2 respectively . Discussion-Identified compounds have anti-inflammatory, analgesic activity used in the treatment of pain related diseases and also in the management of acute and chronic inflammatory diseases. Conclusion-Thus, this GC-MS study indicates the scientific validation of Shoolaprashamana choorna as a potent medicine as claimed by Ayurvedic literature and practice. Keywords: Shoolaprashamana choorna, GC-MS, NIST Library
Publication date: 01/09/2025

https://ijbpas.com/pdf/2025/September/MS_IJBPAS_2025_9379.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.9.9379