ABSTRACT

The roots of Ichnocarpus frutescens of the family Apocyanaceae are known to have several secondary metabolites with antioxidant, anti-inflammatory and anti-cancer activities. During the present study, Lasiodiplodia pseudotheobromae was isolated from the roots of the plant and was identified based on colony morphology, conidial characteristics and ITS1- ITS4 sequences of ribosomal DNA. The ethyl acetate fraction of the endophyte exhibited promising levels of phenolics. The metabolites were analysed by High Resolution Liquid Chromatograph Mass Spectrometer (HR-LCMS). Timnodonic acid, derivatives of indomethacin, matricin, hydroxy cinnamic acid, citraconic acids, furans were predominantly observed. Both the crude and partially purified fractions of endophyte extracts have been found to inhibit lipoxygenase with IC50 values 48 ?g and 55?g respectively. The study reported the presence of indomethacin from the root endophyte for the first time as confirmed by 13C and 1H NMR analysis. Thus, the fungus can be further exploited as a potent candidate to be used against inflammation and serve as an alternative to plant-derived bioactive compounds. Keywords: Ichnocarpus frutescens; Lasiodiplodia pseudotheobromae; HR-LCMS; NMR; anti-inflammatory
Publication date: 01/08/2025

https://ijbpas.com/pdf/2025/August/MS_IJBPAS_2025_9353.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.8.9353