ABSTRACT

The toxin-antitoxins (TA) modules are small genetic operons encoded on the extra chromosomal unit or chromosomal unit of a wide range of bacterial species. The toxin component targets cellular processes like DNA replication, protein translation, and cell wall production resulting in growth arrest of the host cells. However, the presence of antitoxin neutralizes the deleterious action of the toxin component by forming a toxin-antitoxin complex. TA modules have been implicated with several physiological processes such as bacterial survival mechanism against stress, apoptosis, growth arrest, gene regulation, biofilm formation and multidrug tolerance. ParD/ParE module that belongs to a type II toxin antitoxin system is highly abundant in plasmids and bacterial chromosomes. The positively charged toxin ParE can be neutralized by a negatively charged antitoxin ParD resulting in formation of a tight complex. In this study sequence of parD and parE were retrived from the genomic sequence database of Pseudomonas aeruginosa for bioinformatic evaluation and interaction in silico. ParE has several predicted functional partners, including RHH_1 domain-containing protein with score 0.966 and uncharacterized protein with score 0.762. ParD also shows interaction with several predicted functional partners with score 0.966 and SnoaL-like domain- containing protein (0.668). Secondary structures exist as alpha helix, extended strand, beta turn and randomcoil in both the toxin and antitoxin. Molecular docking of ParD antitoxin as ligand against ParE toxin shows involvement of Glu56 of ParE and Val47 of ParD. Valine suggests the involvement of hydrophobic residues for the interaction between the toxin and antitoxin components. Keywords: Toxin; Antitoxin; ParD; ParE; Pseudomonas; TA systems
Publication date: 01/08/2025

https://ijbpas.com/pdf/2025/August/MS_IJBPAS_2025_9293.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.8.9293