ABSTRACT

Low-calorie sweeteners, commonly found globally, are blended together to balance negative flavors. International regulations demand constant monitoring by regulatory authorities and food safety labs of products allowed under different permitted sweetener lists across countries. Identification and quantification of sweeteners require analytical methods capable of affirming analyte identity. These are some examples: Saccharin, acesulfame potassium, aspartame, neotame, and sucralose are various types of sweeteners, which are determined by UV and FTIR as well as by the linearity (Saccharin 50-400 ?g / ml at 266 nm; Aspartame 60-720 ?g / ml at 256 nm;Sucralose 50 to 400 ?g /ml at 260 nm), LOD (Saccharin 5.58 ppm; Aspartame 33.95 ppm; Sucralose 13.5 ppm), LOQ (Saccharin 16.9 ppm; Aspartame 102.8 ppm; Sucralose 41.04 ppm), Range (Saccharin 0.495; Aspartame 1.34; Sucralose 2.87) Precision (Saccharin (0.60 & 0.26); Aspartame (1.96 & 1.911); Sucralose (1.87 & 1.95)), Robustness (Saccharin (265 at 0.71 & 267 at 0.43); Aspartame (at 255 nm is 1.65 & 257 nm is 1.62); Sucralose (259 nm is 0.97 & 261 nm is 0.97), Ruggedness (Saccharin 150?g / ml is 0.43; Aspartame 360 ?g / ml is 1.895; Sucralose 350 ?g / ml is 0.65). And the quantification of sweeteners was done by Beer Lamberts Law, which identified that they were within the limits for marketed products. Keywords: non-nutritive sweeteners, foods, UV-spectroscopy
Publication date: 01/06/2025

https://ijbpas.com/pdf/2025/June/MS_IJBPAS_2025_9139.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.6.9139