ABSTRACT

The study focuses on the isolation and characterization of probiotic bacteria from homemade yogurt and explores the potential health benefits of postbiotics derived from the probiotics. Probiotic bacteria are recognized for their positive effects on gut health, yet their live nature poses certain risks. In general, postbiotics, which include non-viable microbial cells and various metabolites produced during fermentation, have gained attention for their safety and bioactive properties. This research involved the isolation of lactic acid bacteria through a systemic approach, utilizing morphological and biochemical characterization and MALDI-TOF mass spectrometry for accurate identification of the strains. The resulting postbiotic cell-free supernatants were evaluated for their antimicrobial, antioxidant, and antidiabetic activities. The findings revealed substantial antibacterial effects against pathogenic bacteriasuch as Escherichia coli and Salmonella enterica with distinct zones of inhibition correlated to the concentration of the sample. Additionally, antioxidant capacity was quantified through the DPPH assay, revealing the ability to neutralize free radicals. Further, the inhibition of ?-amylase activity was performed, and results indicated potential benefits in carbohydrate metabolism and glycemic control. This study contributes to the therapeutic implications of postbiotics for human health. Keywords: Yogurt, lactic acid bacteria, postbiotics, DPPH and ?- amylase
Publication date: 01/05/2025

https://ijbpas.com/pdf/2025/May/MS_IJBPAS_2025_9061.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.5.9061