ABSTRACT

The anaerobic digestion of organic materials, which is necessary to add chemicals to the continuous biochemical process, is largely mediated by microorganisms. Through a sequence of metabolic events, the microorganisms efficiently break down complex organic compounds to produce simple compounds like methane. In this investigation, inoculums used for biogas production was isolated from agriculture waste sources—L. delbrueckii ssp. Indius AG1 at temperature (35ºC to 37ºC)—to assess their suitability for anaerobic digestion of sterile agriculture waste. The highest biogas production of 215 ml/2 kg sterile waste was obtained using a combination of inoculums about 10ml, and biogas characterization by orsat instrument method showed 47.7% of the methane content. Utilizing morphological, biochemical, and molecular methods, the microbial flora found in the high-yield reactor was identified. The abundance of L. delbrueckii ssp. indius AG1. The study suggests that biogas, which has the potential to displace fossil fuel, is not only a suitable method of managing waste but also a green energy source. Keywords: Nan Sterile agriculture waste, Biogas, L. delbrueckii ssp. indius AG1, Molecular identification, Orsat instrument method
Publication date: 01/05/2025

https://ijbpas.com/pdf/2025/May/MS_IJBPAS_2025_8980.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.5.8980