ABSTRACT

Increasing concerns about fossil fuel availability, greenhouse gas emissions, and pollution caused by the partial combustion of fossil fuels have resulted in a greater emphasis on the use of cellulases to perform enzymatic hydrolysis of lignocellulosic biomass. The primary barrier is the complex structure and composition of lignocellulosic biomass, which are mostly composed of cellulose, hemicellulose, and lignin. Microbes have enzymes capable of breaking down lignocellulose and producing fermentable sugars. The aim of this study was to isolate and screened out potential cellulolytic bacteria from fecal sample of Nilgai (Boselaphus tragocamelus). Based on experiment, total of five bacteria were isolated and out of five only NE bacterial isolate produced clear zone into carboxymethyl cellulose (CMC) agar plate and were identified as cellulase producing bacteria. According to the morphological and biochemical tests, NE bacterial isolate was identified as Pseudomonas sp. NE showed maximum cellulase production (0.061 U/ml) at pH 7.0 after 60 h of incubation at 40°C in a medium containing 1.0% CMC. This bacterial isolate's mesophilic characteristic and ability to produce CMCase supports its potential viability for the present industrial processes that convert lignocellulosic biomass into biofuel. Keywords Cellulase, lignocellulosic biomass, bacterial isolates, characterization, herbivores
Publication date: 01/05/2025

https://ijbpas.com/pdf/2025/May/MS_IJBPAS_2025_8867.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2025/14.5.8867