ABSTRACT

The Himalayan region is predominantly populated by individuals who live in communal settlements. These communities heavily depend on a wide range of plant resources for various purposes, including medicinal uses and other essential needs. In the present investigation, we conducted a comprehensive assessment of the comparative antioxidant efficacy within a specific set of medicinal plant species extracts. In the present study, firstly physoiochemical parameters were performed in both plants as shown in Table 1, the preliminary Phytochemical study for both extracts showed positive and negative results shown in Table 2. The total phenolics content in methanolic extract of Cirisium wallichii exhibited a total phenolics content of 59.25±0.0088 mg Gallic acid equivalent (GAE/g), while the methanolic extract of Fraxinus micrantha showed a total phenolics content of 136.97±0.0058 mg GAE/g. Additionally, the aqueous extract of Cirisium wallichii displayed a total phenolics content of 63.57±0.0088 mg GAE/g, whereas the aqueous extract of Fraxinus micrantha exhibited a total phenolics content of 122.81±0.0066 mg GAE/g. The flavonoid content was recorded in methanolic extract of Cirisium wallichii, as 128.28±0.0190 mg Quercetin equivalent while in the methanolic extract of Fraxinus micrantha, it was measured as 176.52±0.0017 mgQE/g. The aqueous extract of Cirisium wallichii had a flavonoid content of 96.72±0.0300 mgQE/g, whereas the aqueous extract of Fraxinus micrantha contained 149.49±0.0015 mgQE/g of flavonoids. The Tannin content was recorded in methanolic extract of Cirisium wallichii 24.57±0.0016 mg Tannic acidequivalent while in the methanolic extract of Fraxinus micrantha, it was measured as 35.49±0.0012 mgTAE/g. The aqueous extract of Cirisium wallichii had a Tannin content of 11.82±0.0017 mgTAE/g, whereas the aqueous extract of Fraxinus micrantha contained 29.67±0.0015 mgTAE/g of Tannins. The Antioxidant activity of both the extract showed the IC50 by spectrophotometric method by DPPH and ABTS free radical scavenging method. The aqueous extract of Cirisium wallichii and Fraxinus micrantha showed 109.65 ?g/ml and 91.57?g/ml in methanolic 88.02?g/ml and 87.87?g/ml in DPPH. The radical scavenging effect of the aqueous extract of Cirisium wallichii and Fraxinus micrantha showed 120.19?g/ml and 120.00?g/ml, while in methanolic extract it was around 88.02?g/ml for Cirisium wallichii and 94.22?g/ml for Fraxinus micrantha. Both are found to be more potent than that of the synthetic antioxidant ascorbic acid. Cirisium wallichii and Fraxinus micrantha have demonstrated that an increased quantity of phenolic compounds correlates with a heightened efficacy in radical scavenging. Keywords: Himalaya, Hepatoprotective, Uttarakhand, Antioxidant, Flavonoids, Phenolics
Publication date: 01/04/2025

https://ijbpas.com/pdf/2025/April/MS_IJBPAS_2025_8903.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.4.8903