ABSTRACT

Amylases are highly valuable in biotechnology for starch-related industrial activities and are arguably the most studied glycoside hydrolases. Currently, several amylases derived from bacteria and fungus are in use. In the food business, fungal amylases are preferred because they are safe, despite not having the same stability and pH tolerance as their bacterial counterparts. In this context, the objective of the current study was to analyse and compare the antibacterial and biofilm activity of bacterial and fungal amylase commercially produced from Bacillus cereus and Aspergillus oryzae respectively against biofilm producers Staphylococcus aureus MTCC 740 and Pseudomonas aeruginosa MTCC 1688. The antibacterial activity against the test strains by well diffusion method exhibited no significant result with bacterial amylase whereas there was a significant inhibition against P. aeruginosa by fungal amylase with an MIC of 70 ?g/ml. There was also significant reduction in biofilm by both bacterial and fungal amylase with IC 50 37.696 mg/ml and 52.654 mg/ml against S. aureus respectively whereas the observed IC 50 against P. aeruginosa by bacterial and fungal amylase was 37.431 mg/ml and 54.32 mg/ml respectively. The decrease in the virulence factors of P. aeruginosa of both the microbial derived amylases resulted in 70.73% pyocyanin reduction by fungal amylase and 96.45% pigment reduction by bacterial amylase; 1.69% elastase inhibition with fungal amylase and 11.549% elastase inhibition by bacterial amylase was also recorded with the decrease in swarming motility as well. Keywords: Staphylococcus aureus, Pseudomonas aeruginosa, ?-amylase, Las B elastase, pyocyanin, IC 50
Publication date: 15/03/2025

https://ijbpas.com/pdf/2025/March/MS_IJBPAS_2025_MARCH_SPCL_1049.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.3.1049