ABSTRACT

The environmentally sustainable synthesis of nanoparticles is a promising alternative to conventional chemical methods, addressing concerns of environmental toxicity and resource depletion. This study reports the biosynthesis of copper nanoparticles (CuNPs) using Tinospora cordifolia leaf extract (TCLE) as a natural reducing and stabilizing agent. The synthesis process leverages the bioactive compounds in T. cordifolia for the reduction of copper ions, resulting in eco-friendly nanoparticle production. The TC-CuNPs were characterized through DLS, UV- Vis spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) to confirm their structural, optical, and morphological properties. The antimicrobial potential of the synthesized TC-CuNPs was evaluated against antibiotic- resistant microorganisms Mycobacterium tuberculosis. Antimicrobial efficacy was assessed using the Zone of Inhibition (Kirby-Bauer method) assays. The results demonstrated significant antibacterial activity, attributed to the generation of reactive oxygen species (ROS) and the release of copper ions, which disrupt microbial cell membranes and genetic material. The TC- CuNPs exhibited strong inhibitory effects, with larger inhibition zones indicating their potential as effective antimicrobial agents. This study highlighted the use of T. cordifolia as a sustainable resource for nanoparticle synthesis, emphasizing its role in advancing green nanotechnology. The findings pave the way for the development of eco-friendly antimicrobial agents with potential applications in biomedical, pharmaceutical, and environmental sectors, aligning with global sustainability goals. Keywords: Copper nanoparticles, Tinospora cordifolia, Green synthesis, Antimicrobial activity
Publication date: 15/03/2025

https://ijbpas.com/pdf/2025/March/MS_IJBPAS_2025_MARCH_SPCL_1046.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.3.1046