ABSTRACT

Hydrocarbon contamination is a major threat of marine ecosystem. The release of petroleum products by shipping activities and accidental oil spills severely affects the flora and fauna in aquatic environment and result in the imbalance of ecosystem. 5 strains of crude oil degrading bacteria were isolated from the sea water samples of Beypore and Chaliyam fish landing harbours of Kozhikode district. They have shown excellent lipase activity in tributyrin agar plates. The potential of the isolates for crude oil degradation was examined in Bushnell- Haas agar, where crude oil served as the sole carbon source. Selected isolates showed significant growth on the medium. Morphological and biochemical characterization of bacterial isolates was done. The isolates were identified as Bacillus thuringiensis, Cronobacter sakazakii, Proteus vulgaris, Lysinibacillus boronitolerans and Oceanimonas doudoroffii by 16s gene sequencing. Since fish landing harbours are generally more exposed to petroleum products, the isolates obtained from Beypore and Chaliyam are extremely adapted to survive in oily environment with potential lipase activities. The five bacterial strains isolated in this present study have promising potential to be used as bioremediators. Microbial remediation technology is regarded as an eco-friendly and efficient technology. Thus, the products of this present study have application in bioremediation and environmental protection. Keywords: Crude oil, bioremediation, oil spill, lipase activity, marine environment
Publication date: 01/03/2025

https://ijbpas.com/pdf/2025/March/MS_IJBPAS_2025_8830.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.3.8830