ABSTRACT

Recent advancements in Mass spectrometry (MS), which is enable the detection of dozens to hundreds of lipids, tiny chemicals, and protein molecules in a single cell. The ability to describe the cellular heterogeneity in the individual cells is crucial for defining the whole spectrum of cell subtypes and determining their function. In this paper, we address single-cell mass spectroscopy, a methodology that allow for thorough proteome & metabolomic profiling of individual cells using both antibody-free approaches as well as high-throughput, focused mass cytometry-based methodologies. The benefits and drawbacks of various approaches are examined, along with the difficulties and chances for additional advancements in single-cell Mass Spectrometry. Keywords: Cellular Heterogeneity, Lipidomics, Mass Cytometry, Mass Spectrometry, Metabolomics, Proteomics, Single cell analysis
Publication date: 01/02/2025

https://ijbpas.com/pdf/2025/February/MS_IJBPAS_2025_8683.pdf

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https://doi.org/10.31032/IJBPAS/2025/14.2.8683