ABSTRACT

The goal of the present investigation was to isolate Bacillus pumilus KMAS7, a lipase producing bacteria, from edible oil contaminated soil of Cheyyar. The identity of the organism was established by 16S rRNA sequence, in addition to the morphological and biochemical characterization. A mutant strain was developed by random mutation using UV-C radiation. Following mutagenesis and screening, a mutant designated B. pumilus UVM was selected due to its high lipase activity. The selected mutant strain was cultivated in submerged fermentation for lipase production using agriculture weed Argemone mexicana seed oil under different nutritional sources. The maximum lipase of 62.48±0.05 UmL-1 was obtained in the submerged fermentation using 0.5% w/v glucose, 1% w/v ammonium sulphate and 10 ?ML-1 Ni as carbon, nitrogen and trace element sources respectively. Though A. mexicana seed oil has been used for biodiesel production, to our knowledge this is the first report on the lipase production by newly isolated mutant bacterial strain. Keywords: Argemone mexicana, Bacillus pumilus, lipase, submerged fermentation, mutation
Publication date: 01/01/2025

https://ijbpas.com/pdf/2025/January/MS_IJBPAS_2025_8587.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2025/14.1.8587