In current study, UV spectroscopy was performed for estimation of Amlodipine Besilate
(AMLO), Olmesartan Medoxomil (OLME) and Hydrochlorothiazide (HCTZ). Simultaneous
spectrophotometric determination of Amlodipine Besilate, Olmesartan Medoxomil and
Hydrochlorothiazide was carried out by absorbance correction method using UV visible double
beam spectrophotometer. Validation of simultaneous spectrophotometric method was
performed with different parameter like accuracy, precision, recovery, LOD and LOQ. For
absorbance correction method, 258 nm, 315 nm and 356 nm were selected as working
wavelength because at 315 nm and 258 nm Hydrochlorothiazide, at 258 nm Olmesartan
Medoxomil and at 258 nm, 315 nm 356 nm Amlodipine Besilate showed significant
absorbance. Linearity of Amlodipine Besilate, Olmesartan Medoxomil and
Hydrochlorothiazide was found to be 1-6 ?g/mL, 2-24 ?g/mL and 2.5-15 ?g/mL respectively.
The % mean, standard deviation (S.D.), relative standard deviation (% R.S.D.) and standard
error (S.E.) were calculated. The % R.S.D. was less than 2% as required by USP and ICH
guideline. The experimental work involved is very simple, it requires only measurement of
absorbance at selected wavelength. Current study revealed development and validation ofsimple, accurate, precise, rapid, cost effective, reproducible and reliable UV
spectrophotometric method for estimation of Amlodipine Besilate, Olmesartan Medoxomil and
Hydrochlorothiazide in bulk and tablet.
Keywords: UV, validation, analytical method, Amlodipine Besilate, Olmesartan Medoxomil,
Hydrochlorothiazide
Publication date: 01/01/2025
https://ijbpas.com/pdf/2025/January/MS_IJBPAS_2025_8411.pdf
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https://doi.org/10.31032/IJBPAS/2025/14.1.8411
