ABSTRACT

Caesalpinia pulcherrima is a widely documented ethnomedicinal plant that locals have used in varied applications due to its antibacterial, antioxidant, antimutagenic, renoprotective, and other biological activities. The present study investigates the antimitotic and antiproliferative actions of leaf extract of Caesalpinia pulcherrimausing onion (Allium cepa) root tip assay, Vigna radiata shoot inhibition and seed germination assay. In Allium assay and Seed germination assay ethanolic extract of leaves of Caesalpinia pulcherrima showed significant concentration dependant inhibitory influence against the dividing cells of Allium roots and Vigna radiata shoots which was observed by a decrease in root growth, shoot growth and mitotic index as compared to control. A. cepa assay and seed germination assay showed that the Caesalpinia pulcherrima leaf extract can block mitosis by causing spindle and chromosome damage. The extract is genotoxic based on the increasing frequency of chromosomal aberrations at higher concentrations. This study revealed that Caesalpinia pulcherrima crude ethanolic extract has antimitotic and antiproliferative properties. Further study could be performed focusing on identifying and isolating antimitotic, and antiproliferative components of leaves of Caesalpinia pulcherrima and conducting biological screening using other models. Keywords: Caesalpinia pulcherrima; Allium cepa assay; seed germination assay; antimitotic
Publication date: 01/12/2024

https://ijbpas.com/pdf/2024/December/MS_IJBPAS_2024_8445.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.12.8445