FERMENTATIVE PRODUCTION OF ITACONIC ACID BY USING ASPERGILLUS TERREUS Authors: Jayappa K , CHAKRAPANI I S*, KARUNAKAR P, RAJASEKHAR M, INDIRA PRIYADARSINI A AND SRINIVAS N
ABSTRACT
Aspergillus species were known to be the best species for the production of Itaconic acid.
However very little is known about the industrial production of Itaconic acid in India and
virtually nothing is known about its production cost analysis. In view of the extensive industrial
use of Itaconicacid, the present study was designed to increase the production of Itaconic acid
and an attempt was made to optimize the nutrient medium using Aspergillus terreus. Reducing
sugars and phosphorus were measured by the DNS method of Miller (1959) as described by
Strickland and Parson (1972) respectively. The cost-effective substrates like Jatropha seed
cake, ground nut cake, coconut oil cake, glucose, oat meal and corn flour were used as carbon
source for the production of Itaconic acid by using locally isolated Aspergillus terreus strain.
Gene Bank accession number of submitted nucleotide sequence is JF495477. The highest yield
was observed in Jatropha seed cake (13.60 g/lit),followed by Ground nut cake (10.08g/lit),
Coconut oil cake (9.17g/lit), Glucose (7.55 g/lit), Oat meal (4.32 g/lit) and Corn flour (3.27
g/lit).
Keywords: Aspergillus terreus, Itaconic acid, Fermentation, Methylene succinic acid, Secondary Publication date: 01/11/2024 https://ijbpas.com/pdf/2024/November/MS_IJBPAS_2024_8470.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2024/13.11.8470