ABSTRACT

Recent Peptide is promising therapeutics for many diseases. Human Neutrophil is a newer cytolytic broad spectrum antimicrobial peptide, having antibacterial, antiviral, antifungal and anticancer like activity. It has molecular weight of 4 kDa. It is mainly known for its antimicrobial and antiviral activity against huge range of gram-negative, gram-positive bacteria and enveloped, non-enveloped viruses. Peptides are complex bioactive molecule, so it is very challenging to analyse all the parameters for development of peptide. The present work describes analytical method development and validation of Human Neutrophil Peptide-5. An easy, accurate and right method has been developed and validated for estimation of Human Neutrophil Peptide-5 by using BSA as an internal standard, using Enable Q C18 column, water: acetonitrile (60:40 v/v) with Trifluoroacetic acid 0.1% as mobile phase and flow rate of 1 ml/min. Then detection was carried out at 280 nm. The retention time of BSA and HNP-5 was 2.342 and 5.279 min respectively. The linearity range was found to be 10-50 ?g/ml, co-relation coefficient was found to be 0.999. The obtained validation data has been proved that possible utility of the developed method for the estimation of Human Neutrophil Peptide-5. Keywords: Antimicrobial, Peptide, HNP, Antiviral, RP-HPLC, Validation
Publication date: 01/11/2024

https://ijbpas.com/pdf/2024/November/MS_IJBPAS_2024_8467.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.11.8467