ABSTRACT

Diabetes Mellitus (DM) stands as the most whacking chronic metabolic disorder which in turn damage kidney, heart, nerves, eyes and so on. Growing evidence connecting the oxidative stress with DM have authentically suggest antioxidant as a therapeutic agent against DM. Therefore, the present study has evaluated the anti-oxidant potency of lipids extracted from 7 different oleaginous fungi and the most potent antioxidant was further evaluated for its in vitro anti-diabetic potency against RIN5F cells. The free radicle scavenging potency of total lipids was evaluated by DPPH and NO assay. The lipids extracted from Mortierella elongata (Accession No. OK402027) isolated from the Western Ghats of Tamil Nadu was found to be more potent in scavenging free radicles. In vitro lipid peroxidation assay was evaluated against H9C2 cells. In vitro glucose utilization assay against RIN5F cells, Alpha-Amylase Inhibition Assay and Alpha- Glucosidase Inhibition Assay were evaluated to determine the anti-diabetic potency of M. elongata lipids. The present study indicated a significant uptake of glucose when compared with untreated control. Anti-diabetic potency of M. elongata lipids is directly proportional to its concentration. Keywords: PUFA, Anti-diabetic, Antioxidant, NO, DPPH, Lipid Peroxidation
Publication date: 01/11/2024

https://ijbpas.com/pdf/2024/November/MS_IJBPAS_2024_8336.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2024/13.11.8336