ABSTRACT

Struvite stone, which is often associated with urinary tract infection, is one of the types of urinary calculi that affects approximately 8-10% of the global population. The recurrence rate is significantly higher with the treatments that are currently available. So, this study focused on the analysis of the inhibitory potential of synthesized zinc oxide nanoparticle using the petals extract of Rosa centifolia on the gel grown struvite crystals as well as their antimicrobial activity against the urinary tract infection causing pathogens. Additionally, their antioxidant activity was also explored. The synthesized zinc oxide nanoparticle was characterized by UV- Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction (XRD), scanning electron microscopy (SEM) and Energy dispersive X ray (EDX). The growth of gel grown struvite crystal was analysed by subjecting them with different concentration of synthesized zinc oxide nanoparticle. This revealed that the growth of the crystal was gradually decreased from 1.45cm to 0.45cm on increasing the concentration from 1% to 5% respectively. Then the antimicrobial activity of synthesized zinc oxide nanoparticle against major urinary tract infection causing pathogens such as Escherichia coli(3cm), Klebsiella pneumonia(4cm), Enterococcus faecalis(3cm), Staphylococcus aureus(4cm), Candida albicans(4cm) and Candida vulgaris(3cm) was examined by measuring zone of inhibition in cm using disc diffusion method. The antioxidant activity also recorded in terms of IC50 which is 21.7% using DPPH assay. As a result, this study confirms that synthesized zinc oxide nanoparticle using the petals extract of Rosa centifolia has antiruolithiatic, antimicrobial and antioxidant activity on gel grown struvite crystals. Keywords: Medicinal plants, Rosa centifolia, Green synthesis, Nanotechnology, Zinc oxide nanoparticle, Urinary tract infection, Urinary stone, Struvite crystals, Urolithiasis, antimicrobial activity, antioxidant activity
Publication date: 01/11/2024

https://ijbpas.com/pdf/2024/November/MS_IJBPAS_2024_8219.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2024/13.11.8219