CYTOTOXICITY STUDY OF WATER SAMPLES COLLECTED FROM LOCAL TRIBUTARIES BY ALLIUM CEPA TEST Authors: Nikam SA , POWAR PV AND MAVCHI UU
ABSTRACT
Urban effluents may contain several cytotoxic contaminants that are dumped into rivers,
causing environmental degradation in downstream reaches. Rivers in Pune (Maharashtra) are
the most polluted water bodies because of their function in transporting waste from
municipalities and industries as well as run-off from agricultural fields in their basin. Despite
different restrictions and regulations, a variety of small-scale companies discharge their trash
directly into the river. The current work employed an Allium cepa bioassay to assess cell
toxicity by water samples collected from polluted bodies of water in Pune as a result of
industrial effluent. The study was carried out by collecting wastewater samples from three
sampling sites (Site A - Bhima River, Site B - Pavna River, and Site C - Indrayani River) and
investigating their physicochemical properties. Tap water was used as a control in every case.
The water quality of the samples was evaluated using a TDS meter (Total Dissolved Solids).
Water cytotoxicity was assessed using the Allium cepa bioassay. The study's findings revealed
that A. cepa bulbs exposed to Indrayani River water had a considerable reduction in MI,
followed by Pavna and Bhima River water. The Indrayani and Pavna Rivers frequently get a
higher volume of domestic and industrial sewage from several municipalities than the Bhima
River. The findings of this investigation reveal that the river water pollutants comprised several
toxic compounds that are harmful to the aquatic environment and human health.
Keyword: Allium cepa, Cytotoxic, Mitotic index, Pollution, TDS meter, Water Publication date: 01/11/2024 https://ijbpas.com/pdf/2024/November/MS_IJBPAS_2024_8030.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2024/13.11.8030