ABSTRACT

This research aims to establish an UV-Visible spectroscopic technique to develop an easy, precise, sensible, and reproducible method for Metadoxine by applying a multivariate regression equation. Proposed technique depends on the equation of the linear regression performed by taking absorbance at five distinct wavelengths. Metadoxine’s maximum absorbance was obtained at 324 nm. Graph obtained from concentration 14-26 ?g mL-1 resulted in linear curve and the regression coefficient was obtained as 0.9999. % RSD values for Intra- day, as well as Inter-day precision, was obtained as 0.965733 and 0.349379. The assay value determined was between 99.78% - 99.90% w/w. Keywords: Metadoxine, Hepatoprotective agent, UV spectrophotometry, Multivariate calibration, Assay, ICH guidelines
Publication date: 01/10/2024

https://ijbpas.com/pdf/2024/October/MS_IJBPAS_2024_8387.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.10.8387