ABSTRACT

The detection and measurement of biomolecules and metabolites in animal and human tissues utilizing bimolecular approaches requires the adoption of a procedure. The bioanalytical approach is an accurate way to count the quantity of substances and metabolites that are present in a biological system. The development of new techniques, the verification of already established protocols, and the examination of specimens are among the most important aspects of bioanalysis. First and foremost, a molecule may be measured using many ways, and it can be recognized using a variety of analytical approaches. Extraction methods such as protein precipitation in complicated plasma and biological samples, liquid extraction, and solid-phase extraction are some of the ways that drugs may be examined for quality control purposes. The linearity, specificity, sensitivity, reproducibility, accuracy, selectivity, and recovery of an assay are the validation factors that are taken into consideration. In order to contribute to the process of ensuring the high quality of pharmaceuticals, we propose the creation and validation of bioanalytical systems. Keywords: LC-MS/MS, Bioanalytical technique, Extraction techniques, Method validation
Publication date: 01/10/2024

https://ijbpas.com/pdf/2024/October/MS_IJBPAS_2024_8351.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.10.8351