ABSTRACT

The objective of this study was to assess the in vitro anti-inflammatory and the antioxidant properties of Shorea robusta and Acorus calamus. The anti-inflammatory properties of the methanolic extracts Shorea robusta and Acorus calamus were assessed through two in vitro tests namely hemolysis inhibition and protein denaturation inhibition. The results showed that both plant extracts inhibited protein denaturation in a concentration-dependent manner. At a concentration of 1000g/ml, the value of inhibition of protein denaturation of methanol extract of Acorus calamus and Shorea robusta was 83.99 ?g/ml. When used in combination, the two plant extracts significantly increased the prevention of hemolysis and denaturation of proteins. Antioxidants are essential for reducing oxidative stress, which is a primary cause of many chronic illnesses. In this investigation, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) tests were used to measure the antioxidant activity. Both the extracts scavenged the DPPH free radicals in a concentration-dependent manner. Interestingly, the percentage of the combination of both plant extracts was higher than ascorbic acid. The present study of methanolic extracts Acorus calamus and Shorea robusta exhibited potent anti-inflammatory and antioxidant properties and can be considered as a synergistic drugs during their use in traditional herbal medicine. Keywords: Shorea robusta, Acorus calamus, anti-inflammatory, antioxidant activity
Publication date: 01/10/2024

https://ijbpas.com/pdf/2024/October/MS_IJBPAS_2024_8307.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.10.8307