ABSTRACT

Background: 2,6-dichloro indophenol is the most common textile dye used across the globe for dyeing textile fibers. The textile industries discharge untreated effluents and are let into city drains that enter rivers and lakes. This brings out changes in pH, Total Organic Carbon, Biological Oxygen Demand, Chemical Oxygen Demand, and other water quality parameters are affected. Several physical and chemical methods are employed for the removal of the contaminating dye from wastewater such as chemical precipitation, adsorption, reduction, sonication, electrochemical treatment, etc. Methods: In the present study, 2,6-dichloro indophenol has been used for the dye degradation analysis using fungi isolated from the soil. The study focuses on the extent of decolorization of the dye at various concentrations by inoculating with fungi isolate F. equiseti. This work is to understand the effectiveness of Fusarium equiseti in the degradation of 2,6-dichloro indophenol. The decolorization of the dye was monitored by UV-Visible spectrophotometer analysis. The FTIR analysis further substantiated the degradation of 2,6-dichloro indophenol. Results: After performing spectroscopic analysis on the 2nd, 4th, and 6th day of incubation, the dye showed a maximum of 75.23% degradation within 6 days with a dye concentration of 0.02%. UV-Visible spectrophotometer analysis demonstrated an absorption peak at 500 – 550 nm for dye decreased gradually with the increase of exposure time indicating a degradation reaction. The presence of a weak band was observed at 3281 cm -1 corresponding to O-H stretching alcohol and new medium bands were formed during the incubation suggesting the conversion of alcohol in the presence of Fusarium equiseti to alkane. The study of enzyme assay is to measure the Pectinase activity using Oswald’s Viscometer, showing the reduction in the viscosity after 2 hours indicating the increase in the pectinase activity with the dye and fungal culture. The pH study shows maximum degradation at pH 4. Keywords: Dye degradation, 2,6-dichloro indophenol, Fusarium sp, Decolorization, Bioremediation
Publication date: 01/10/2024

https://ijbpas.com/pdf/2024/October/MS_IJBPAS_2024_8206.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.10.8206