ABSTRACT

In this study DOE based approach is used to prepare and characterized acyclovir loaded liposomes. Different ratios of phospholipid along with cholesterol and different methods of preparation were evaluated by studying various properties of drug loaded liposomes. Acyclovir loaded liposomes are prepared using Phospholipon 90H along with cholesterol. Liposomes are prepared by using three different methods i.e. Ethanol Injection, Film Hydration and Reverse- Phase Evaporation Method. Entrapment efficiency, zeta potential and PDI are three evaluation parameters selected in this study to do detailed statistical analysis and optimization of acyclovir loaded liposomes. Total 24 different batches based on 2 level central composite design were prepared using three different methods of preparation and having different ratios of lipid and cholesterol. Results shows that acyclovir loaded liposomes prepared film hydration method using a ratio of 1:0.15 of HSPC and Cholesterol a maximum entrapment efficiency of 62.3 %, particle size of 91.38 nm and PDI of 0.142. Keywords: Design of experiment, Liposomes, Acyclovir, Film hydration, Ethanol injection, Reverse phase evaporation
Publication date: 01/09/2024

https://ijbpas.com/pdf/2024/September/MS_IJBPAS_2024_8304.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.9.8304