ABSTRACT

Salbutamol Sulphate and Budesonideare available in the market as a combination for the treatment of asthma. The developed chromatographic system depends on usingHiQ sil C8 column (4.6 mm x 250 mm). The mobile phase was prepared by mixing Acetonitrile: 0.02M of Phosphate buffer (pH 3) 50:50v/v at a flow rate of 1 ml/min detection at 228 nm, the injection volume 20 ?l. The method satisfied linearity with regression coefficient R20.9965 for Salbutamol Sulphateand R20.9993 for Budesonide in the range 5-25 ?g/ml for Salbutamol Sulphate and 2-10 ?g/ml for Budesonide. TheLOD and LOQ; were 0.23 and0.70?g/ml for Salbutamol Sulphate and 0.02 and0.06?g/ml for Budesonide. The method showed satisfying results. Keywords: Validation, Combination, Salbutamol Sulphate,Budesonide, HPLC
Publication date: 01/09/2024

https://ijbpas.com/pdf/2024/September/MS_IJBPAS_2024_8282.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.9.8282