ABSTRACT

The aim of this work was to develop and validate a simple estimation method for Lamivudine in Active pharmaceutical dosage form using UV spectroscopic method. The method was developed using distilled water as a solvent and absorbance was measured at 270 nm. Beers law was obeyed the concentration range of 5 – 25 ?g/ ml. Calibration curve shows a linear relationship between the absorbance and concentration. The line equation y =0.375x+0.004 with correlation coefficient (r) of 0.9998 was obtained. The method was validated as per ICH guidelines. The method was validated statistically and by recovery studies. The percentage recovery was found to be in the range between 98.09 and 101.46%. The %RSD value was found to be less than 1. A simple, accurate and cost-efficient spectroscopic method has been developed for the estimation of Lamivudine in active pharmaceutical dosage form. Keywords: Lamivudine; Distilled water; Validation; ICH Guidelines, UV Spectroscopy
Publication date: 01/08/2024

https://ijbpas.com/pdf/2024/August/MS_IJBPAS_2024_8274.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.8.8274