ABSTRACT

Context- A simple, sensitive, accurate and reproducible spectrophotometric method has been developed. The first order derivative approach was used for Simultaneous estimation curcumin and ascorbic acid. Objective- Methanol was employed as a solvent for the development of spectrophotometric method. Materials and methods- For the measurement of Ascorbic acid, the first order derivative absorption at 422.0 nm (zero crossing point of Curcumin) was used, and for the quantification of Curcumin, the first order derivative absorption at 261.0 nm (zero crossing point of Ascorbic acid) was employed. Curcumin and Ascorbic acid linearity was established over concentration ranges of 2.4-7.2 g/ml and 10-30 g/ml, respectively, with correlation coefficients (r2) of 0.9999 and 0.9987. The % assay for formulation was found to be 99.89%±0.0500 for Curcumin and 100.39%±0.170 for Ascorbic acid by the proposed method. Mean% recoveries of Curcumin and Ascorbic acid is 99.76%-100.44% and100.09%- 100.40%, respectively. Results- The approach was found to be repeatable by analyzing the results of Repeatability, Reproducibility and intermediate precision. LOD and LOQ were 0.30and 0.99 ?g mL-1 for Curcumin, 4.2 and 12.6 ?g mL-1 for Ascorbic acid. Conclusion- According to the recovery investigation, the approach can be successfully applied to pharmaceutical formulation with no interference from excipients. All of the validation parameters were within acceptable limits. Keywords: Ascorbic acid, Curcumin, Development, Spectrophotometric, Validation
Publication date: 01/08/2024

https://ijbpas.com/pdf/2024/August/MS_IJBPAS_2024_8240.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.8.8240