The Rosa alba L. is a little-large tree or shrub up to 1.8 m in height with wide branches and
thorns. It belongs to the family Rosaceae. The purpose of the present study was to ascertain the
pharmacognostic, cytomorphological, and chromatographic studies of the flowers of R. alba.
Microscopic studies of the flower among its different parts like a petal, sepal, pollen grains, anther,
ovule, and its powdered drug were performed by routine methods. Results of the microscopic
studies reported the occurrence and features of various cellular parts like sclereids, trichomes,
crystals, oil globules, vascular bundles, etc. Heavy metals and pesticides in the powdered drug
were determined by atomic absorption spectroscopy and gas chromatography equipped with an
electron capture detector (GC-ECD) respectively. Nickel, chromium, iron, zinc and cadmium
heavy metals were found in the powdered drug but no pesticides were found. A very prompt and
precise reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode
array detection (DAD) was used for the simultaneous estimation of quercetin, gallic acid, ?-
sitosterol and vanillin in the hydroalcoholic extract of the flowers. These phytoconstituents were
separated from the extract through the C18 column (4.6 mm x 250 mm) using methanol:
acetonitrile: water (40: 15: 45, v/v/v) along with 1% acetic acid as mobile phase. Quercetin, Gallicacid, ?-sitosterol, and vanillin were found to be 0.2179, 1.8937, 0.9991, and 0.0065% w/w
respectively in the ethanolic (85%, v/v) extract of the flower of R. alba.
Keywords: Rosa alba, GC-ECD, RP-HPLC, Heavy metal, Pesticide, AAS
Publication date: 01/08/2024
https://ijbpas.com/pdf/2024/August/MS_IJBPAS_2024_8226.pdf
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https://doi.org/10.31032/IJBPAS/2024/13.8.8226
