ABSTRACT

Introduction- Chromatography is used for both qualitative and quantitative drug examination, especially for mother tinctures and to check the purity and identify any adulterants that may be present. Objective - To evaluate the quality of the Homoeopathic Pharmaceutical Company's Eupatorium perfoliatum Mother Tincture utilising its fingerprinting properties. Methodology- The qualitative analysis of Eupatorium perfoliatum Mother Tincture Using High Performance Thin Layer Chromatography (HPTLC) has been performed to assess the quercetin content. Results- Analysis using High Performance Thin Layer Chromatography (HPTLC) revealed that the Rf value of quercetin was about 0.5 in all the three samples. The regression equation was foundto be Y1 (sample A) = 0.0009x+ 0.0016, Y2(sample B) =0.0009x+0.0021, Y3(sample C) = 0.0008+0.0029 with respective to concentration. Additionally, a correlation coefficient of 1.0 suggests that the relationship between concentration and area is linear. Conclusion- The quercetin content was found in all the three samples of mother tinctures, but sample C was considered to be standard (R2 = 0.9979). Keyword: HPTLC, Qualitative analysis, Eupatorium perfoliatum, Mother tincture, Quercetin-3- glucoside
Publication date: 01/07/2024

https://ijbpas.com/pdf/2024/July/MS_IJBPAS_2024_8188.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.7.8188