ISOLATION AND CHARACTERIZATION OF BACTERIOCIN PRODUCING BACTERIA ISOLATED FROM ORAL CAVITY
Authors: Ranpariya D , ANDHARE P, BHATTACHARYA I, THAKUR A AND UPADHYAY D*

ABSTRACT
The mouth is a diverse ecosphere that houses a huge range of microorganisms such as fungi, viruses and bacteria. Oral cavity consists of a variety of habitats, each with its own intrinsic, particular mini-environment, such as tonsils, hard and soft palates, tongue, cheeks, and gingival sulcus. Plaque biofilms on dental surfaces and planktonic phase organisms floating in saliva are also examples of oral microbiome organisms. The majority of oral bacteria have not yet been cultured; however, culture-independent techniques have been effective in providing a through description of the mouth bacterial ecology. The dental flora is constantly altering because its interaction with the surroundings and produces bacteriocins and competes for nutrients within this tiny ecosystem. The objective of the current research was to evaluate and contrast the microbial flora of both healthy and unhealthy people. In this study, the isolation and identification of bacteriocin-producing bacteria was carried out. The samples were collected from Parul University Master's students in Vadodara, Gujarat, India. The bacteria were extracted and identified using microscopy, gram staining, and biochemical analysis. The study resulted in the isolation of 5 different bacterial strains, of which 2 were gram negative, whereas three were gram positive. The very dominant species belonged to the Streptococcus and Escherichia species as revealed according to Gram staining and biochemical tests. Many other bacteria were also isolated such as Staphylococcus, enterococcus, Bacillus subtilis family etc. It was found that the Enterobacter and Lactobacillus species produced bacteriocin against Escherichia coli. Some of these microbes have been connected to the emergence of multiple infectious illnesses. Keywords: Bacteriocin, fungi, virus, bacteria, Streptococcus pneumoniae, Escherichia coli, Staphylococcus, enterococcus, Lactobacillus
Publication date: 01/07/2024
    https://ijbpas.com/pdf/2024/July/MS_IJBPAS_2024_8139.pdf
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https://doi.org/10.31032/IJBPAS/2024/13.7.8139