ABSTRACT

The previous few decades have seen a surge in petroleum products. This pollution led to significant problems. Microorganisms are used in bioremediation process to clean petroleum contamination, it is able to break down petroleum hydrocarbons. The current research sought to identify the microorganisms found in oil-contaminated sites from two different geographic locations (Vadodara and Mandavi (Surat), Gujarat, India), to examine the development of isolated bacteria and their properties. The research used the enrichment culture technique to isolate bacteria. Bushnella-Hass (BH) broth supplemented with crude oil as a sole carbon source. Based on morphological and biochemical characterization, the isolates were identified as Bacillus spp. The isolates were morphologically identified as gram’s negative, short rod shaped and red in colour. The growth of bacterial population showed by growth curve. Biodegradation assay were performed at lowest (1%) and highest (10%) concentration for 78 hrs by using selected bacterial culture. The ability of isolates to degrade oil was 52% done by S1 and 60% done by S2 assessed using the gravimetric analysis. In growth condition, maximum growth was optimized at 1% inoculum size after incubation and in optimization test maximum growth was enhance at pH 8.0 and temperature 35 ? after 48 hrs incubation. Antibiotics sensitivity test performed for bacterial growth, isolates were responsive to drugs and have no antagonise impact of soil-friendly microorganisms. The current study was to isolate, characterise, and assess the bacterial population's capacity to degrade oil. This study aims to make some recommendations for the development of bacterial remediation. Keywords: Bioremediation, Potential, Petroleum Hydrocarbon, Hydrocarbon degradation, antagonise impact
Publication date: 01/07/2024

https://ijbpas.com/pdf/2024/July/MS_IJBPAS_2024_8137.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2024/13.7.8137