ABSTRACT

The present work is aimed to develop and characterize silver nanoparticles obtained by green synthesis from n- hexane leaf extract of Pachygone ovata (Poir.) and evaluate their cytotoxic potential in B16F10 cells and fibroblast (3T3) cell lines. In this study, AgNP morphology and size of the obtained nanoparticle were characterized by UV-visible (UV-vis) absorption spectroscopy, SEM-EDS for elemental composition and zeta potential (ZP), XPS and XRD analysis. The synthesized nanoparticles showed maximum absorbance at 420 nm according to UV–vis spectroscopy results at 24 hours' time. Silver nanoparticles and their quasi-spherical shapes were obtained with an average size of 19nm. Perform cell cycle analysis to elucidate the mechanism of cytotoxicity in B16F10 cells. The aqueous extract of Pachygone ovata (Poir.) Leaf extract was found to be effective in the environmentally friendly synthesis of silvernanoparticles. Silver nanoparticles with spherical shapes, small sizes, and uniform properties were successfully synthesized. The nanoparticles produced had a significant impact on cell viability reduction. Metal nanoparticles are thought to have caused apoptosis, or cell death, in some cells. Keywords: Pachygone ovata (Poir.) leaf extract, AgNPs, Characterization, MTT, B16F10 cells and fibroblast (3T3) cell lines, anti-cancer activity
Publication date: 01/06/2024

https://ijbpas.com/pdf/2024/June/MS_IJBPAS_2024_8091.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.6.8091