ABSTRACT

Snehakalpana (preparations with fats), a commonly used Ayurvedic dosage form, allows active ingredients to be absorbed into fatty media. To address the lack of data for fat-based formulations, Yashtimadhu Ghrita (YG) was formulated and analyzed using HPTLC to estimate glycyrrhizin content. Various tests were conducted on raw drugs and prepared ghee. The pH, refractive index, specific gravity, acid value, saponification value, and iodine value of YG were determined as 6-7, 1.457, 0.912, 1.792, 145.66, and 41.467 respectively. Phytochemical analysis showed the presence of carbohydrates, cardiac glycosides, saponins, and tannins in chloroform and hexane extracts of YG. After developing an HPTLC quantitative analysis method using the chloroform extract after trial and errors, glycyrrhizin was estimated at 1.506 mg in 50 mg of YG's chloroform extract, accounting for over 50% incorporation from the drug (514.2 mcg in 10 mg of GG's alcoholic extract) into the media. The Rf value of the medicated ghee obtained at 254 nm with glycyrrhizin as the standard was 0.331 ± 0.021. The coefficient of variation was 41.07%. The study acknowledges the importance of HPTLC as a precise analytical tool for determining the active constituents of drugs. The formulation and analysis of YG provide valuable insights into the quantitative estimation of glycyrrhizin in a fat-based formulation, contributing to the knowledge base for such Ayurvedic formulations. Keywords: Chromatography, glycyrrhizin, quantification, Yashtimadhu Ghrita
Publication date: 01/06/2024

https://ijbpas.com/pdf/2024/June/MS_IJBPAS_2024_8076.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.6.8076