ABSTRACT

The cytogenetic effects of organophosphate pesticide chloropyrifos 20% EC on root meristem of Allium cepa L. were studied. The bulbs with roots of Allium cepa L. were treated with different concentration of chlorpyriphos (0.2%, 0.4%, 0.6%, 0.8%,1.0%) for 24, 48 and 72 hours. Acetocarmine squash technique was adopted to prepare the slide of root tips of Allium cepa L., after getting a certain length. To measure the cytotoxic level mitotic index was prepared of microscopic observation of the cytological slide. The result indicated that Chlorpyriphos significantly increase the genetic abnormality in different concentration and treatment period when compared with control plant. Obtained data shows statistically significant difference between treated and control root meristem of Allium cepa L. (P < 0.05). All concentration and treatment period of chlorpyriphos induced a number of chromosomal aberrations in root tip cells of Allium cepa L. Among various chromosomal aberration, chromatin bridge, breaks, stickiness, laggard, vagrant, fragments, C-mitosis, multipolarity, ring chromosome, as well as micronuclei were observed in mitotic preparation. The RAR and PAC was also augmented. Pesticide shows that it was highly toxic to plant cell. Keywords: Chlorpyriphos, Cytotoxicity, Mitotic Index, Chromosome abnormality, Allium cepa L.
Publication date: 01/06/2024

https://ijbpas.com/pdf/2024/June/MS_IJBPAS_2024_8070.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.6.8070