ABSTRACT

The AcrAB efflux pump of E.coli is comprises of an RND- type transporter AcrB and a accessory protein AcrA. Efflux pump inhibitors are captivating compounds which helps in reversing multidrug resistance in bacterial pathogens. In the present study we tested the ability of rutin a flavonoid to inhibit AcrAB efflux pump of E.coli. A ethidium bromide accumulation assay and berberine assay study suggest that rutin is capable of inhibiting the AcrAB efflux pump. Rutin inhibits the pump at a concentration of 1000?g/ml. only ciprofloxacin and tetracycline MICs were reduced to two fold in presence of rutin at a concentration of 1000?g/ml. Since rutin considerably enhances accumulation of ethidium bromide in ethidium bromide accumulation assay. From the previous reports we suggest that ability of efflux pump inhibitors to restore the susceptibility to different antibiotics is highly substrate specific. Keywords: AcrAB, Efflux pump, MIC, Rutin
Publication date: 01/05/2024

https://ijbpas.com/pdf/2024/May/MS_IJBPAS_2024_8024.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.5.8024