ABSTRACT

Bacillus licheniformis SCJ4, a marine bacteria isolated from highly polluted area of Lucknow, showed a high potency for contemporaneous production of alkaline protease enzyme. In the current study, Plackett- Burman factorial design was applied to evaluate culture conditions affecting the production of enzyme. Analysis of Plackett-Burman design results revealed that, the most significant variables affecting alkaline protease production were glucose, K2HPO4, MgSO4 and CaCl2 with more than 6 folds increase in enzyme activity than basal medium (1185 Uml-1min-1 after 48h). Keywords: Bacillus licheniformis, fractional factorial design, enzymes
Publication date: 01/04/2024

https://ijbpas.com/pdf/2024/April/MS_IJBPAS_2024_7963.pdf

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https://doi.org/10.31032/IJBPAS/2024/13.4.7963